Features and Benefits

Compatible with high-throughput handling systems.

General description

Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 µM citrate for colorimetric assays and 0.5 to 40 µM for fluorimetric assays.

Principle

In this kit, citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 µM citrate for colorimetric assays and 0.5 to 40 µM for fluorimetric assays.

Suitability

Suitable for citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)